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. 2018 Aug 7;17:122. doi: 10.1186/s12934-018-0971-2

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Improving the cellulase activity through linker optimization, engineering the secretory pathway and increasing the scaffoldin length for efficient cellulosome assembly. Activity of a β-glucosidase, b exoglucanase and c endoglucanase without and with different linkers between enzyme and dockerin. d Engineering secretory pathway improved Tr-L-dBGL1 extracellular activity. e Engineering secretory pathway improved PYD1-L-dBGL1 extracellular activity. f FACS analysis of surface display of scaffoldin ScafAGA5. Data were the mean values of two repeats ± standard deviation