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Mutation of the three Nkx2–5 sites ablates Tolloid-like 1 (Tll- 1) activation by Nkx2.5. QCE-6 cells were transfected with Tolloid- like 1 (Tll-1) with the proximal (P), medial (M), or distal (D) Nkx2–5 sites mutated with or without the cotransfection of Nkx2–5. Data are expressed as relative luciferase units (RLU) ± SEM.
