Table 1.

Characteristics of patients with faecal samples submitted for diagnostic testing that yielded positive, equivocal or low level amplification Strongyloides sp. 18S rRNA real-time PCR compared to confirmatory testing using a second nested Strongyloides sp. 18S rRNA PCR with SSCP and sequencing. Values in bold are positive/above the normal range; * same patient; ^ another specimen on the same patient was PCR negative; Eos: eosinophil; nd; not done; SSCP: single strand conformation polymorphism; NW: North West; NTH: North; CW: Central West.

Age (Years)	Gender	Place of Residence	Region	Real-time PCR	Mean Ct Value	Nested PCR	Sequence Identity/SSCP Homology	Strongyloides Serology	Eosinophil Count (×109/L)	Parasites Detected by FEAC Microscopy
2	F	Doomadgee *	NW	Positive	18.77	Detected	S. stercoralis	nd	nd	S. stercoralis
2	F	Doomadgee *	NW	Positive	19.16	Detected	S. stercoralis	nd	0.57	S. stercoralis
51	F	Doomadgee	NW	Positive	23.72	Detected	S. stercoralis	Reactive (3.1)	0.43	S. stercoralis
41	F	Doomadgee	NW	Positive	25.66	Detected	S. stercoralis	Reactive (1.6)	0.93
2	F	Doomadgee *	NW	Positive	31.13	Detected	S. stercoralis	nd	3.69	S. stercoralis
9	M	Doomadgee	NW	Positive	35.25	Detected	H. nana	nd	2.84	S. stercoralis, H. nana, B. hominis, E. nana
70	M	Aurukun *	NW	Positive	27.44	Detected	S. stercoralis	nd	0.89	B. hominis
70	M	Aurukun *	NW	Positive	28.00	Detected	S. stercoralis	nd	0.72	B. hominis
28	M	Mount Isa	NW	Positive	28.10	Detected	S. stercoralis	nd	nd
66	M	Mount Isa	NW	Positive	36.27	Detected	B. hominis	nd	0.02	B. hominis
30	M	Mount Isa	NW	Positive	36.41	Detected	S. stercoralis	nd	0.41
61	M	Magnetic Island	NTH	Positive	31.15	Detected	S. stercoralis	nd	nd
28	M	Magnetic Island	NTH	Positive	35.69	Detected	S. stercoralis	nd	0.30
64	F	Magnetic Island	NTH	Positive	35.93	Detected	Human gene	nd	0.60
51	F	Magnetic Island	NTH	Positive	36.12	Detected	S. stercoralis	nd	nd	B. hominis
36	M	Townsville	NTH	Positive	31.86	Detected	S. stercoralis	Reactive (4.0)	1.27	B. hominis
70	M	Townsville	NTH	Positive	34.12	Detected	S. stercoralis	nd	0.13
40	M	Townsville	NTH	Positive	36.10	Detected	S. stercoralis	Reactive (3.1)	1.48	H. nana, B. hominis, Entamoeba coli
<1	F	Townsville	NTH	Positive	36.13	Detected	S. stercoralis	nd	0.00
58	M	Townsville	NTH	Positive	36.57	Detected	S. stercoralis	nd	0.10
<1	M	Townsville	NTH	Positive	37.08	Detected	S. stercoralis	nd	nd
14	F	Townsville	NTH	Positive	37.31	Detected	S. stercoralis	nd	nd
39	F	Townsville	NTH	Positive	38.51	Not Detected		nd	0.08
69	F	Townsville	NTH	Positive	38.97	Detected	Candida sp.	nd	0.46
51	F	Townsville	NTH	Positive	39.33	Detected	Candida sp.	nd	0.20
43	F	Jundah	CW	Positive	34.32	Not Detected		nd	0.04	B. hominis
67	F	Mornington Island	NW	Positive	35.08	Detected	B. hominis	Reactive (4.1)	2.14
1	M	Mornington Island	NW	Positive	36.96	Detected	Human gene	nd	8.04	G. intestinalis
35	M	Normanton	NW	Positive	35.81	Detected	S. stercoralis	nd	1.90
36	M	Palm Island	NTH	Positive	36.53	Detected	S. stercoralis	nd	0.25
<1 y	M	Palm Island	NTH	Positive	38.37	Not Detected		nd	nd
1	M	Palm Island ^	NTH	Positive	38.48	Detected	Human gene	nd	nd
20	F	Palm Island	NTH	Positive	38.84	Detected	S. stercoralis	nd	0.62
78	M	Ayr	NTH	Positive	36.63	Detected	S. stercoralis	nd	0.14
1	M	Cloncurry	NW	Positive	36.81	Not Detected		nd	nd	B. hominis, E. nana
94	F	Home Hill	NTH	Positive	36.95	Not Detected		nd	0.12	B. hominis
80	F	Greenvale	NTH	Positive	37.01	Detected	S. stercoralis	nd	0.09
11	F	Charters Towers	NTH	Positive	37.15	Detected	S. stercoralis	nd	nd
79	M	Hughenden	NW	Positive	37.66	Detected	Saccharomyces sp.	nd	1.05
27	M	Camooweal	NW	Positive	38.12	Detected	Saccharomyces sp.	nd	0.25	G. intestinalis
84	M	Ingham	NTH	Positive	38.40	Not Detected		nd	0.09	B. hominis
63	F	Ingham	NTH	Positive	38.53	Not Detected		nd	0.09