Figure 1.
Vector descriptions. pVgEcR-MP, retroviral vector for constitutive expression of a modified Drosophila ecdysone receptor (containing the VP16 transactivation domain) from the LTR and marked with the puromycin-resistance gene; pRXR-MN, retroviral vector for constitutive expression of the RXR from the LTR and marked with the neomycin (G418)-resistance gene; pI-TK Hygro, self-inactivating retroviral ecdysone-inducible vector marked with HYG; pI-GFPsin, same vector as pI-TK Hygro but without hygromycin-selectable marker; pI-LacZ: pI-TK Hygro containing the β-galactosidase gene. U3-R-U5, LTR; gag, envelope gene; VgEcR, ecdysone receptor; PGK, phosphoglucokinase promoter; TKp, enhancerless thymidine kinase promoter; E/GRE, hybrid ecdysone response element (5×); Hsmin, minimal heat shock promoter; PURO, puromycin resistance gene; NEO, G418 resistance gene; MCS, multiple cloning site; GFP, green flourescent protein; LacZ, β-galactosidase gene.
