Skip to main content
. 2001 Oct 30;98(23):13043–13048. doi: 10.1073/pnas.221450598

Figure 3.

Figure 3

(A) Dose–response of PTEN-inducible line to muristerone A. Western blot analysis of dose–response for cell line, U87.23.WT, expressing hemagglutinin-tagged wild-type PTEN. Cells were induced with doses of muristerone A, in increments of 0.1 μM, from 0 (lane 2), to 1.5 μM (lane 17), for 30 h. For comparisons, lane 1 shows a cell line with inducible β-galactosidase (negative control) treated with 1.5 μM muristerone A, and lanes 19 and 20 show endogenous levels of PTEN in two PTEN-positive human cell lines, 293T, and HeLa respectively. The filter was probed by using anti-PTEN C terminus antibodies. Background expression is undetectable, even after very long exposures, and maximal expression is perhaps only a few fold elevated over endogenous levels. (B) PTEN and LY alter the cell growth pattern of U87MG cells in low serum in a similar fashion. U87MG cells carrying wild-type or mutant (G129E) inducible PTEN were seeded in 2% FBS DMEM at high density and either induced to express PTEN with 0.5 mM muristerone A (Upper Left and Upper Center), or left uninduced (Lower Left and Lower Center panels). U87MG cells were treated with 10 μM LY294002 for 24 h (Upper Right) or untreated (Lower Right). Nomarski photographs were taken 24 h after induction.