Table 2. Repeat instability in Gc-r clones from cell lines with the CGG153-HyTK cassette is accompanied by an elevated frequency of complex mutations in the reporter as compared to CGG53- and CGG0-HyTK cassettes combined.

(A) Mutation patterns, including mutations of (G)₇ and (C)₆ runs in the TK domain in sequenced Gc-r clones; (B) mutation patterns without mutations of (G)₇ and (C)₆ runs in sequenced Gc-r clones. Statistical significance of the differences was verified by Fisher standard test. Treatment of the clone carrying CGG153-HyTK cassette with of Pold3 siRNA wipes out complex mutations.