KEY RESOURCES TABLE.
| Reagent or resource | Source | Identifier |
|---|---|---|
| Antibodies | ||
|
| ||
| Hts-RC | Developmental Studies Hybridoma Bank | htsRC |
| GFP | Developmental Studies Hybridoma Bank | 12A6 |
| Kelch | Developmental Studies Hybridoma Bank | kel1B |
| HA | ThermoFisher Scientific | 71-5500 |
| DE-Cadherin | Developmental Studies Hybridoma Bank | DCAD2 |
| β-catenin pTyr142 | ECM Biosciences | CP1081 |
| FasIII | Developmental Studies Hybridoma Bank | 7G10 |
| pTyr clone 27B10 | Cytoskeleton | APY03 |
| Anti-tubulin | Developmental Studies Hybridoma Bank | E7 |
|
| ||
| Critical Commercial Assays | ||
|
| ||
| RNeasy Plus Mini kit | Qiagen | Cat No. 74134 |
| QuantiNova SYBR Green RT-PCR kit | Qiagen | Cat No. 208152 |
| Clarity Western ECL substrate | BioRad | Cat No. 1705060S |
|
| ||
| Experimental Models: Organisms/Strains | ||
|
| ||
| MTD-GAL4 P{w[+mC]=otu-GAL4::VP16.R}1, w[*]; P{w[+mC]=GAL4-nos.NGT}40; P{w[+mC]=GAL4::VP16-nos.UTR}CG6325[MVD1] |
Bloomington Drosophila Stock Center | 31777 |
|
matαTub-GAL4 w[*]; P{w[+mC]=matalpha4-GAL-VP16}V2H |
Bloomington Drosophila Stock Center | 7062 |
|
matαTub-GAL4 w[*]; P{w[+mC]=matalpha4-GAL-VP16}V37 |
Bloomington Drosophila Stock Center | 7063 |
|
UAS-msn-RNAi y[1] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.HMJ02084}attP40 |
Bloomington Drosophila Stock Center | 42518 |
| Misshapen-YFP w[1118]; PBac{802.P.SVS-2}msn[CPTI003908] |
KYOTO Stock Center (DGGR) | 115454 |
| Cheerio-YFP w[1118]; PBac{602.P.SVS-1}cher[CPTI000847] |
KYOTO Stock Center (DGGR) | 115123 |
| w1118;;UAS-Myr-HA-MsnWT | L. Xu | |
| w1118;;UAS-HA-MsnWT | This paper | |
| w1118;;UAS-HA-MsnKR | This paper | |
| w1118;UAS-Myr-HA-MsnKR | This paper | |
|
| ||
| Oligonucleotides | ||
|
| ||
| Misshapen qRT-PCR | ||
| 5′-TCCCTTGGACAGCAGCGATT-3′ | ||
| 5′ – AGTTCCATCGTTCCTAGCC – 3′ | ||
| GAPDH qRT-PCR | (Giuliani et al., 2014) | |
| 5′ – AAATCGCGGAGCCAAGTAGT - 3′ | ||
| 5′ – CACGATTTTCGCTATGGCCG – 3′ | ||
|
| ||
| Recombinant DNA | ||
|
| ||
| pMK33 with Myr-HA-MsnWT | L. Xu | |
| pENTR3c Dual with HA-MsnWT | This paper | |
| pENTR3c Dual with HA-MsnKR | This paper | |
| pENTR3c Dual with Myr-HA-MsnKR | This paper | |
| UASp-pPW with HA-MsnWT | This paper | |
| UASp-pPW with HA-MsnKR | This paper | |
| UASp-pPW with Myr-HA-MsnKR | This paper | |
|
| ||
| Software and Algorithms | ||
|
| ||
| Fiji/ImageJ | https://fiji.sc/ | |
| Origin | OriginLab Corp | Version 6.1 |
| LASX | Leica | Version 3.3.3.16958 |
|
| ||
| Other | ||