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. Author manuscript; available in PMC: 2018 Aug 8.
Published in final edited form as: Cell Rep. 2018 Jun 5;23(10):3021–3030. doi: 10.1016/j.celrep.2018.05.010

Figure 1. Obesity/IR Targets the MSRN, Promotes Macrophage Cholesterol Loading, and Exacerbates Atherosclerosis.

Figure 1

(A–F) “Obesity/IR only” model: WT C57BL/6 mice were fed a low-fat diet (LFD) or high-fat diet (HFD) for 9 weeks. “HC ± obesity/IR” models: Ldlr−/− mice were fed a chow diet, a Western-type diet (WTD), or a high-cholesterol-low-fat (HCLF) diet for 12 weeks.

(A) Metabolic parameters.

(B) Proteomics analysis of peritoneal macrophages. Differentially abundant proteins (yellow, up; blue, down) were identified based on the G-test (G > 1.5) and t test (p < 0.05).

(C) Oil-red-O staining of peritoneal macrophages.

(D) Cholesterol levels in peritoneal macrophages treated with/without 2% serum from WTD-fed Ldlr−/− mice.

(E) Cholesterol levels in peritoneal macrophages.

(F) Aortic root lesion area.

Results are mean ± SEM. *p < 0.05 (t test). n = 5–10 mice/group. See also Figures S1 and S2 and Table S1.