(A and B) Peritoneal macrophage mRNA levels for several genes involved in cholesterol metabolism in WT C57BL/6 mice fed a LFD or a HFD (“obesity/IR only” model, A) or in Ldlr−/− mice fed chow, a WTD, or a HCLF diet (“HC ± obesity/IR” models, B).
(C) Peritoneal macrophage media protein levels for APOE and C3.
(D) Peritoneal macrophages were isolated from 8-week-old WT, Apoe−/−, or C3−/− C57BL/6 mice fed a LFD. Macrophages were treated were with and without 2% serum from WTD-fed Ldlr−/− mice, and cholesterol levels were quantified.
Results are mean ± SEM.
*p < 0.05 (t test). n = 5 mice/group.