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. Author manuscript; available in PMC: 2018 Aug 8.
Published in final edited form as: CNS Neurol Disord Drug Targets. 2014;13(9):1571–1579. doi: 10.2174/1871527313666140806125841

Fig. 2. HIV/gp120 evokes a short-time activation of Beclin-1.

Fig. 2

Cortical neurons were exposed to gp120 (5 nM) for various times. Lysates were prepared in RIPA buffer and Beclin-1 was analyzed by Western blot on 4–12% NuPAGE SDS gel. 1=DMSO; 2=boiled gp120; 3=gp120 1 hour; 4=gp120 3 hours; 5=rapamycin 3 hours; 6=gp120 6 hours. Results were replicated in three independent experiments.