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. Author manuscript; available in PMC: 2018 Aug 8.
Published in final edited form as: CNS Neurol Disord Drug Targets. 2014;13(9):1571–1579. doi: 10.2174/1871527313666140806125841

Fig. 3. HIV/gp120 promotes LC3-I conversion to LC3-II.

Fig. 3

Neurons were treated with 5nM gp120 for the indicated times. Total cell lysates in RIPA buffer were analyzed by Western blot on 4–12% NuPAGE SDS gel using monoclonal anti-LC3 antibody. The level of LC3-II was increased relative to LC3-I after 3 hours of treatment (n=3) but LC3-II levels returned to control at 18 hours, suggesting autophagic induction up to 3 hours and an autophagosome clearance at 18 hours.