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. 2001 Oct 23;98(23):13055–13059. doi: 10.1073/pnas.231364798

Figure 5.

Figure 5

In vivo angiogenic activity secreted by fibrosarcoma cells. Hydron pellets were prepared containing buffer (PBS), fibrosarcoma culture-conditioned medium (FS), tumor cell medium treated with anti-PLF-related protein mAb 28.1.1, which does not recognize PLF (FS28), or tumor cell medium from which PLF had been removed by immunodepletion with mAb 209 (FS209). The pellets were placed in rat corneas, and neovascularization was monitored after 7 days. Vigorous ingrowth of the new vessels from the limbus (L) in the direction of the pellet (P) was scored as neovascularization (N). Inverted grayscale images are shown to enhance visualization of neovascularization. Positive responses were detected in 0/4 corneas with PBS, 4/4 with FS, 3/4 with FS28, and 0/4 for FS209.