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Apparent affinity of cyto c for the binding to inner mitochondrial membranes plotted as a function of pH (filled circles). As described by Kawai et al., these data were obtained from double-reciprocal plots of respiration rates versus the total concentration of cyto c added to the medium. The data were fitted by using a modified version of the Henderson-Hasselbach equation. Taken from ref. (Kawai et al. 2009) with permission
