Fig. 14.

Fluorescence anisotropy of 2 μM zinc substituted cytochrome c (λex = 423 nm, λem = 588 nm, [cyt^o c] = 3 μM) with added TOCL/DOPC liposomes as a function of total lipid concentration. The addition of 150 mM NaCl to a sample with a lipid concentration of 660 μM leads to a decrease in the ZnP anisotropy value as indicated. The line resulted from a fit to the Hill binding equation with Ka 1.5∙10⁴ M⁻¹. Taken from ref. (Hanske et al. 2012) with permission