Fig. 6.
(Left) Quenching of the pyrene monomer emission (RFI) from PPGPC induced by the binding of cyto to egg PC vesicles (LUVs) containing 17.6 mol% cardiolipin. The vesicle mixture contained different mixtures of pyrene labeled and unlabeled liposomes. The corresponding molar ratios of labeled to unlabeled liposomes were 1:1 (open square), 1:5 (open circle), 1:10 (open triangle, pointing up), and 1:20 (open triangle, pointing down). (Right) Change of fluorescence quenching due to the addition of unlabeled liposomes with 17.6 (filled square and +), 33.3 (filled circle), and 100 (filled rhombus). The upward arrow indicates the gain in fluorescence due to the addition of 150 mM NaCl. The lipid concentration indicated on the abscissa refers to the concentration of phosphate groups. The + data points were obtained for a cytochrome c concentration of 123 nm, at which non-labeled liposomes with 17.6 mol% CL were added. The figure was taken form ref. (Rytömaa and Kinnunen 1995)