Table 1.
Human tankyraseankyrase (TNKS/TNKS2) ARC constructs for biophysical assays. The proteins include a non-native, vector-derived GAMGS sequenceTankyrase Binders at the N-terminus that is retained upon cleavage of the affinity tag [7]
| ARC construct | Construct boundaries | Molecular weight (kDa) |
|---|---|---|
| TNKS ARC1–5 | 178–958 | 85.0 |
| TNKS ARC1 | 178–336 | 17.2 |
| TNKS ARC2–3 | 331–645 | 34.7 |
| TNKS ARC4 | 646–807 | 18.0 |
| TNKS ARC5 | 799–958 | 17.5 |
| TNKS2 ARC1–5 | 20–800 | 85.3 |
| TNKS2 ARC1 | 20–178 | 17.7 |
| TNKS2 ARC2–3 | 173–487 | 35.3 |
| TNKS2 ARC4 | 488–649 | 17.9 |
| TNKS2 ARC5 | 641–800 | 17.3 |