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. Author manuscript; available in PMC: 2019 Feb 15.
Published in final edited form as: J Immunol. 2018 Jul 11;201(4):1211–1221. doi: 10.4049/jimmunol.1701122

Figure 1. BMDM phagocytosis of target B cells is dependent on BMDM saturation status.

Figure 1

(A) BMDM were loaded with AF488 labelled 0.4-260 beads per BMDM and the extent of phagocytosis was assessed by flow cytometry and microscopy. Histogram show AF488 intensity when BMDM are empty (0.4 beads per BMDM) or full (260 beads beads per BMDM). (B) Increasing the amount of bead per BMDM increased BMDM loading with plateaux above 26:1 (* denote statistical significance when compared to 260 beads per BMDM condition). (C) Differentially bead loaded BMDM were incubated together with target human CD20 transgenic murine splenic B cells opsonised with Ritm2a or an Irrevelant antibody (Irr) and percentage of bead and B cell positive BMDM, (* denote statistical significance when compared to 0 beads per BMDM condition) and (D) B cell associated fluorescence after subtraction of Irr-B cell fluorescence (* denote statistical significance when compared to 0 beads per BMDM condition) is shown. Means of 4-5 independent experiments, error bar: mean±SD, analysed by ANOVA.