Table 1. Repeat sequences of the ssDNA materials.
| Namea | Repeat block sequencesc |
|---|---|
| p(A20-i) | [AAAAAAAAAAAAAAAAAAAA-TTAGGATAGATATACGGGTTC]12-50 |
| p(T20-j) | [TTTTTTTTTTTTTTTTTTT-GATTTTAGAGGATCGTGTGGTTTTAC]10-60 |
| p(A20-i-XL) | [AAAAAAAAAAAAAAAAAAAA-TTAGGATAGATATACGGGTTC-CGGATGCGCATCCG]7-14 |
| p(T20-k-XL) | [TTTTTTTTTTTTTTTTTTTT-CTAAGTACAGTCAGGTAAGCTA-CGGATGCGCATCCG]6-16 |
| p(C20-l)b | [CCCCCCCCCCCCCCCCCCCC-CGAGATGTCACT]2-10 |
| p(G20-l)b | [GGGGGGGGGGGGGGGGGGGG-CGAGATGTCACT]4-20 |
| p(A20-l)b | [AAAAAAAAAAAAAAAAAAAA -CGAGATGTCACT]2-10 |
a
i, j, k, l are barcode sequences that allow functionalization and are used to localize dyes with complementary strands. XL is a palindromic sequence for self-hybridizing duplex formation.
b
Synthesised via ligation rather than RCA, because RCA cannot be performed due to the strong CG base pairing (and their unusually high content).
c
The lower case number denotes the range of degree of polymerization obtained from gel electrophoresis (Supplementary Note 1, Supplementary Fig. 1).