Figure 1. Psychedelics Promote Neuritogenesis both In Vitro and In Vivo.
(A) Chemical structures of psychedelics.
(B) Representative tracings of cortical neurons (DIV6) treated with compounds.
(C) Sholl analysis demonstrates that psychedelics increase dendritic arbor complexity (n = 39–41 neurons).
(D) Area under the curve (AUC) of the Sholl plots in (C).
(E) Maximum number of crossings (Nmax) of the Sholl plots in (C).
(F–I) Cortical neurons treated with psychedelics display an increase in the number of branches (F), the number of primary dendrites (G), and the total length of the dendritic arbor (H) but not the length of the longest dendrite (I).
(J and K) Class I neurons from Drosophila larvae treated with psychedelics during the first instar display increased branching (J) but not total length of the dendritic arbor (K) (n = 3 neurons).
(L) Representative images of neurons from (J) and (K).
(M and N) Class I neurons from Drosophila larvae treated with psychedelics during the third instar display increased branching (M) but not total length of the dendritic arbor (N) (n = 3 neurons).
(O) Representative images from (M) and (N).
*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, as compared to vehicle control (VEH). Scale bars, 30 μm. Data are represented as mean ± SEM. See also Figures S1–S5.