Fig. 5.

Intron mobility assays comparing the TS34 hairpin with 5′-exon hairpins having continuous Watson-Crick base pairs. (a) Predicted secondary structures of the TS34 hairpin and a subset of 5′-exon hairpins comprised of continuous Watson-Crick base pairs from other GsI-IIC target sites in the G. stearothermophilus strain 10 genome. (b) Mobility efficiencies of the target sites containing the 5′-exon hairpins shown in (a). Mobility assays were performed with recipient plasmids containing the 5′-exon from the indicated target site combined with the same TS23 3′ exon. The target sites were cloned in the LAG recipient plasmid, and mobility efficiencies were determined by the ratio of (Tet^R+ Amp^R)/(Amp^R) colonies, as described in Fig. 4. The bar graphs show the mean and standard deviation for 3 independent experiments.