Skip to main content
. Author manuscript; available in PMC: 2019 Jun 7.
Published in final edited form as: Curr Protoc Nucleic Acid Chem. 2018 Jun 7;73(1):e54. doi: 10.1002/cpnc.54

Figure 1. Schematic of AuSAXS workflow to determine gold label-gold label distance distributions.

Figure 1

Scattering intensity equation for a single-labeled molecule. The scattering signal can be decomposed into a sum of the individual scattering contributions: double-labeled sample, the macromolecule only, two gold-macromolecule cross-terms, and the interference from the gold labels. Schematic of the workflow to determine the Au-Au distance distribution. The SAXS profiles of the shown samples are used to extract the gold-gold interference scattering profile. The interference pattern is Fourier transformed into a distance distribution using basic profiles generated with the size distribution of Au nanocrystals.