Fig. 6.

Representative immunofluorescence microscopy analysis of in vitro differentiated Mks from cord blood at day 13 of culture (red = β1-tubulin; blue = nuclei; scale bar = 25 μm) (Ai). Representative immunofluorescence analysis of proplatelet formation and structure from cord-blood derived Mks in adhesion on type IV collagen-coated glass coverslips (red = β1-tubulin; blue = nuclei; scale bar = 10 μm) (Aii). Percentage of CD41⁺CD42b⁺ in vitro differentiated Mks from cord blood hematopoietic progenitors (B). Schematic representation of 3D Mk culture (C). Representative confocal microscopy imaging of a lyophilized silk sponge upon CFSE⁺ Mk seeding (scale bar = 200 μm) (Di). Greater magnification of the area contained within the white box highlighting Mks adhering onto silk scaffold. Arrows indicate CFSE⁺ Mk (green = Mks; blue = silk; scale bar = 50 μm) (Dii)