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. Author manuscript; available in PMC: 2019 Jun 15.
Published in final edited form as: ACS Chem Biol. 2018 May 30;13(6):1668–1676. doi: 10.1021/acschembio.8b00313

Figure 4.

Figure 4

Flow cytometry analysis showed that immunization with Qβ-MUC1 conjugates 10–13 induced IgG antibodies capable of binding with a panel of MUC1 expressing tumor cells stronger than sera from mice immunized with Qβ only. Mean fluorescence intensities of binding to (a) RMA-MUC1 cells; (b) B16-MUC1 cells; and (c) MCF-7 cells. Binding was tested with 1:20 dilutions of the post-immune sera. * p < 0.05, ** p < 0.01, *** p < 0.001. The p values were determined through a two-tailed t-test using GraphPad Prism.