Table 1. Staining panel for surface markers.
| Marker | Metal | Clone | Dilution |
|---|---|---|---|
| Anti-PE | 156Gd | PE001 | 1:50 |
| CD3 | 170Er | UCHT1 | 1:100 |
| CD4* | 146Nd | RPA-T4 | 1:200 |
| CD7 | 153Eu | CD7-6B7 | 1:100 |
| CD8a* | 145 Nd | RPA-T8 | 1:50 |
| CD16 | 148Nd | 3G8 | 1:100 |
| CD20* | 163Dy | 2H7 | 1:200 |
| CD25 | 149Sm | 2A3 | 1:100 |
| CD27 | 167Er | O323 | 1:100 |
| CD28* | 171Yb | CD28.2 | 1:100 |
| CD38 | 172Yb | HIT2 | 1:200 |
| CD45 | 89Y | HI30 | 1:100 |
| CD45RA | 169Tm | HI100 | 1:100 |
| CD45RO* | 173Yb | UCHL1 | 1:100 |
| CD49b* | 176Yb | P1e6c5 | 1:100 |
| CD69 | 144Nd | FN50 | 1:50 |
| CD103* | 155Gd | Ber-ACT8 | 1:50 |
| CD107 (LAMP)* | 143Nd | H4A3 | 1:50 |
| CD122* | 158Gd | TU27 | 1:50 |
| CD126 (IL6R)* | 154Sm | UV4 | 1:40 |
| CD127 | 165Ho | AO19D5 | 1:200 |
| CD152 (CTLA4)* | 166Er | 14D3 | 1:40 |
| CD161 | 164Dy | HP-3G10 | 1:100 |
| CD196 (CCR6) | 141Pr | G034E3 | 1:100 |
| CD197 (CCR7) | 159Tb | G043H7 | 1:100 |
| CD223 (LAG) | 150Nd | 874501 | 1:40 |
| CD278 (ICOS) | 151Eu | DX29 | 1:50 |
| CD279 (PD-1) | 175Lu | EH 12.2H7 | 1:100 |
| CD335 (NKp46)* | 174Yb | 9E2 | 1:50 |
| CD336 (Nkp44)* | 147Sm | P44-8 | 1:50 |
| CD357 (GITR)* | 142Nd | 621 | 1:40 |
| HLA-DR* | 168Er | L243 | 1:200 |
| TCRgd | 152Sm | 11F2 | 1:50 |
| KLRG-1* | 160Gd | REA261 | 1:50 |
*self-conjugated