Fig 6. Gp95-induced Ca²⁺ hyperactivity in feline cultured hippocampal neurons.

Representative traces of GCaMP5 fluorescence intensity and a summary graph of normalized average of total Ca²⁺ activity in (i) control neurons and neurons treated with (ii) 1-nM gp95, (iii) 200-nM AMD3100, (iv) 200-nM AMD3100 and 1-nM gp95, (v) 10-μM RP, (vi) 10-μM RP and 1-nM gp95, (vii) 10-μM CNQX, and (viii) 10-μM CNQX and 1-nM gp95, showing that the gp95-induced Ca²⁺ hyperactivity in cat hippocampal neurons is dependent on CXCR4, cGKII, and AMPARs (n = number of neurons, ****p < 0.0001, one-way ANOVA, uncorrected Fischer’s LSD, F (7,260) = 5.296). A scale bar indicates 20 seconds. AMD3100, bicyclam derivative plerixafor hydrochloride; AMPAR, AMPA receptor; cGKII, cGMP-dependent protein kinase II; CNQX, 6-Cyano-7-nitroquinoxaline-2,3-dione; LSD, Least Significant Difference.