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. 2018 Jul 27;14(7):e1007230. doi: 10.1371/journal.ppat.1007230

Fig 6. RtkS (SMDB11_2269) is a post-translational activator of T6SS activity.

Fig 6

(A) T6SS-dependent Hcp secretion as measured by immunoblot detection of Hcp in cellular and secreted fractions of wild type (WT) or mutant (ΔtssE, ΔppkA, ΔpppA, ΔrtkS, ΔppkAΔrtkS and ΔpppAΔrtkS) strains of S. marcescens Db10. (B,D) T6SS-dependent anti-bacterial activity as determined by recovery of target organisms P. fluorescens or S. marcescens ATCC274 following co-culture with strains of S. marcescens Db10 (mutants as above, together with ΔtagFΔrtkS, ΔppkAΔtagF and ΔppkAΔtagFΔrtkS, as indicated). Individual data points are overlaid with mean +/- SEM. (C) Representative images of wild type (WT) and ΔrtkS mutant strains of S. marcescens Db10 expressing TssB-GFP and Fha-mCherry fluorescent fusions. In the lower three panels these strains are further carrying the vector control plasmid (+VC, pSUPROM) or a plasmid directing the expression of RtkS in trans (+ RtkS, pSC590). Panels show individual fluorescence channels (TssB-GFP, Fha-mCherry) and an overlay of the fluorescence channels and the DIC channel (Merge; GFP signal false-coloured green and mCherry red). Scale bar, 5 μm.