Extended Data Figure 11. In vivo histidine supplementation sensitizes tumors to methotrexate without enhancement of treatment toxicity (part 2).

a, b. Histological analyses indicated that the kidney and liver appeared normal at the end of the two week recovery period. See also Supplementary Figure 3a “toxicity test_extra panels”, top panel. a. H&E analyses of kidney sections from mice from all experimental groups. Tissues were collected at the conclusion of the experiment, after two weeks recovery post treatment. Group sizes are the same as in Extended Data Fig. 9a. b. H&E analyses of liver sections from mice from all experimental groups. Group sizes are the same as in Extended Data Fig. 9a. Tissues were collected at the conclusion of the experiment, after two weeks recovery post treatment. See also Supplementary Figure 3b “toxicity test_extra panels”, bottom panel: No differences in the overall tissue morphology between methotrexate-treated mice and mice treated with methotrexate and histidine supplementation in intestines collected immediately at the end of a 5 day treatment. H&E analyses of intestine sections from mice from all experimental groups of mice bearing SEM tumors xenografts. These samples were collected immediately after the conclusion of a 5 day-therapy regime of daily injections, to allow the detection of acute damage to the intestine following the treatment and before recovery. This data show no difference in immediate damage to the intestine of mice treated with either methotrexate alone or methotrexate in combination with histidine supplementation.