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. Author manuscript; available in PMC: 2018 Aug 8.
Published in final edited form as: Nat Methods. 2018 Apr 3;15(4):233–234. doi: 10.1038/nmeth.4642

Figure 1.

Figure 1

Simulated expression and RNA-seq read counts for 40 genes in which the last 10 genes (A–J) are differentially expressed across two phenotypes (−/+). Simulated quantities and heat maps are log-scaled. (a) Simulated log mean expression levels for the genes generated by sampling from the normal distribution with mean 4 and s.d. 2. In the + phenotype the differential expression of genes A–J was created by the addition of a standard normal to each mean expression in the – phenotype. (b) The simulated RNA-seq read counts for ten subjects in each phenotype generated from an overdispersed Poisson distribution based on mean expression in a with biological variation. The heat map shows z-scores of the read counts normalized across all 20 subjects for a given gene.