STAR*METHODS.
KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-Rabbit phospholipase A2 | Abcam | Cat#ab135825 |
| anti-Mouse beta-Actin | Sigma-Aldrich | Cat#A5441; RRID: AB_476744 |
| Goat anti-Rabbit IgG-HRP | Santa Cruz Biotechnology | Cat#sc-2054; RRID: AB_631748 |
| Goat anti-Mouse IgG-HRP | Santa Cruz Biotechnology | Cat#sc-2005; RRID: AB_631736 |
| anti-Digoxigenin-AP, Fab fragments | Roche | Cat#11093274910 |
| Bacterial and Virus Strains | ||
| wt PA14 | Joao Xavier (MSKCC) | N/A |
| eGFP-tagged PA14 | Joao Xavier (MSKCC) | N/A |
| PA14 exoU | Feinbaum et al., 2012 | N/A |
| PA14 fleN | Feinbaum et al., 2012 | N/A |
| PA14 ORF_11 | Feinbaum et al., 2012 | N/A |
| One Shot TOP10 Chemically Competent E. coli | Thermo Fisher | Cat#C404010 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Arachidonic acid | Sigma | Cat#A9673 |
| Acetylsalicylic acid (Aspirin) | ACROS Organics | Cat#AC158180025 |
| Cycloheximide | Sigma | Cat#01810 |
| Digitonin | Cayman | Cat#14952 |
| Dimethyl sulfoxide | Sigma | Cat#276855 |
| ethyl 3-aminobenzoate methanesulfonate | Sigma | Cat#10521 |
| Gentamycin | IBI Scientific | Cat#IB02030 |
| Licofelone | Cayman | Cat#10007692 |
| Agarose LM | Gold Biotechnology | Cat#A-204-100 |
| Melittin | Millipore | Cat#444605 |
| Zileuton | Cayman | Cat#10006967 |
| Nitrotetrazolium Blue chloride (NBT) | Sigma-Aldrich | Cat#N6876 |
| 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt (BCIP) | Sigma | Cat#B8503 |
| Paraformaldehyde | Aldrich | Cat#441244 |
| Methanol | Fisher | Cat#A452-4 |
| Formamide DI deionized | AmericanBio | Cat#AB00600 |
| Maleic acid | Sigma-Aldrich | Cat#M0375 |
| Sodium chloride | Fisher | Cat#BP358-212 |
| Trizma base | Sigma | Cat#T1503 |
| Glycerol | Sigma | Cat#G2025 |
| Critical Commercial Assays | ||
| mMessage mMachine SP6 Kit | Thermo Fisher | Cat#AM1340 |
| RevertAid First Strand cDNA Synthesis Kit | Thermo Fisher | Cat#K1621 |
| PhosphoProtein Purification Kit | QIAGEN | Cat#37101 |
| QuikChange Lightning Site-Directed Mutagenesis Kit | Agilent | Cat#210518 |
| DIG RNA Labeling Kit | Roche | Cat#11175025910 |
| Deposited Data | ||
| mRNA sequencing data | This paper | GEO: GSE111528 |
| Experimental Models: Organisms/Strains | ||
| Zebrafish: wt casper | White et al., 2008 | N/A |
| Zebrafish: TG(lysC:Casper1GR) | This paper | N/A |
| Zebrafish: TG(hsp70:cPla2mKate2) | Enyedi et al., 2016 | N/A |
| Sequence-Based Reagents | ||
| Forward primer for S498A mutagenesis: 5′- TCAACCCTGC CCTTCGCTCCCTTCAGCGGCATC – 3′ | Enyedi et al., 2016 | N/A |
| Reverse primer for S498A mutagenesis: 5′ – GATGCCGCT GAAGGGAGCGAAGGGCAGGGTTGA – 3′ | Enyedi et al., 2016 | N/A |
| cpla2 splice MO1: 5′- AAGCGTCACTTACTATAATGTTGGA-3′ | Enyedi et al., 2013 | N/A |
| cpla2 splice MO2: 5′-TCCCTTTTAGAATTTACCTTGCCGA-3′ | This paper | N/A |
| cpla2 ATG MO: 5′-ATGCTCAACTATAATGTTGGACATT-3′ | Enyedi et al., 2013 | N/A |
| pycard MO: 5′-CAATTGCACTTACATTGCCCTGTGT-3′ | Progatzky et al., 2014 | N/A |
| myd88 MO: 5′-TAGCAAAACCTCTGTTATCCAGCGA-3′ | van der Sar et al., 2006 | N/A |
| myd88 MisPrime MO: 5′-TAcCAtAACCTgTGTTATCgAGgGA-3′ | van der Sar et al., 2006 | N/A |
| Forward primer to confirm the knockdown of pycard MO: 5′ -CGCGTCACAAAGTCTGCAAT-3′ | Progatzky et al., 2014 | N/A |
| Reverse primer to confirm the knockdown of pycard MO: 5′ -CATCAGAGGGAGCACCTTTGC-3′ | Progatzky et al., 2014 | N/A |
| Forward primer to generate il1b antisense probe: 5′- ATGGC ATGCGGGCAATAT-3′ | This paper | N/A |
| Reverse primer to generate il1b antisense probe: 5′- TAATA CGACTCACTATAGGGCTGCAGCTCGAAGTTAATGATG-3′ | This paper | N/A |
| Software and Algorithms | ||
| ImageJ/FIJI | Schindelin et al., 2012 | https://fiji.sc/ |
| MTrackJ plugin of ImageJ/FIJI | Meijering et al., 2012 | https://imagescience.org/meijering/software/mtrackj |
| MOSAIC plugin of ImageJ/FIJI | Sbalzarini and Koumoutsakos, 2005 | http://mosaic.mpi-cbg.de/?q=downloads/imageJ |
| MATLAB | Mathworks Inc., USA | http://mathworks.com |
| Anaconda Python 2.7 | Anaconda Inc., USA | https://www.anaconda.com |
| CellProfiler | Lamprecht et al., 2007 | http://cellprofiler.org |
| Minitab | Minitab Inc., USA | http://www.minitab.com |
| Prism 7 | GraphPad Software | https://www.graphpad.com/scientific-software/prism |
| Other | ||
| TRIzol Reagent | Life technologies | Cat#15596026 |
| FluoSpheres polystyrene, 1.0 µm, blue-green (430/465) | Life technologies | Cat#F13080 |
| PhosSTOP EASYpack | Roche | Cat#04906837001 |
| cOmplete Tablets EASY pack | Roche | Cat#04693116001 |
| NuPage 4-12% Bis-Tris Protein Gels | Thermo Fisher | Cat#NP0321BOX |
| Dynabeads Oligo(dT)25 | Invitrogen | Cat#61002 |
| RIPA Buffer | Cell Signaling | Cat#9806S |
| Glass beads, acid-washed 425-600 µm | Sigma-Aldrich | Cat#G8772 |
| Proteinase K | Roche | Cat#03115879001 |
| Saline sodium citrate (SSC) buffer, 20X solution | AmericanBio | Cat#AB13156 |
| Tween-20 | Sigma | Cat#P1379 |
| Bovine Serum Albumin (BSA) | Sigma | Cat#A2153 |
| Fetal Bovine Serum (FBS) | Corning | Cat#35-010-CV |
| Heparin sodium salt from porcine intestinal mucosa | Sigma-Aldrich | Cat#H4784 |
| Ribonucleic acid from torula yeast | Sigma | Cat#R6625 |
| Phosphate buffered saline (PBS) | Sigma | Cat#79382 |
| 2.0 mL MT Graduated Mixed tube | Fisher Scientific | Cat#05-408-146 |
| Amersham ECL Western Blotting Detection Reagents | GE Healthcare | Cat#RPN2106 |