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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: J Immunol. 2018 May 18;201(1):243–250. doi: 10.4049/jimmunol.1800236

Figure 3. CBirRag T cells proliferate normally and respond specifically to CBir1 flagellin, which is found in all mouse strains used in this study (A and B).

Figure 3

CFSE-labeled CBirWt splenocytes were cultured with splenic CD11c+ dendritic cells for 4 days and stimulated with either (A) 10ng/mL CBir1 peptide or supernatant from boiled fecal pellets of indicated mouse strains or (B) fecal pellets from Rag1−/− mice orally treated with vancomycin. (C) Splenocytes from CBirWt, CBirTCR and CBirRag mice were labeled with CFSE and cultured for 4 days with splenic CD11c+ dendritic cells and 10ng/mL CBir1 peptide. Shown is one representative experiment of three independent experiments