Fig. 3. Evaluation of the five purified IgG antibodies for their binding activity to the native forms of GP expressed on the cell surface. One microgram per mililiter of the IgG antibodies from clones, D36-1, D11-3, D12-1, D34-2, and E140-2 were reacted with 5×105 HEK293-GP and HEK293 cells, followed by incubation with FITC-conjugated anti-mouse IgG (Fc) for flow cytometry. Thin line: control IgG, Thick line: experimental IgG. GP, glycoprotein; FITC, fluorescein isothiocyanate.
