Table 1. The binding properties of the purified IgG and IgM antibodies from 7 hybridoma clones.
| Name | Types | Binding activity to 5 GP1s (by ELISA) | Binding activity to cell-surface GP (by flow cytometry) | The lowest GP-binding concentrations (by ELISA) (ng/mL) |
|---|---|---|---|---|
| C36-1 | IgG2a, κ | No binding to 5 GP1s | - | 1.593 |
| D11-3 | IgG1, κ | No binding to 5 GP1s | + | 7.8 |
| D12-1 | IgG1, κ | GP1e (MLR) | + | 7.8 |
| D34-2 | IgG1, κ | No binding to 5 GP1s | - | 1.593 |
| E12-10 | IgM | ND | ND | ND |
| E49-3 | IgM | ND | ND | ND |
| E140-2 | IgG1, κ | No binding to 5 GP1s | + | 1.593 |
5 GP1s indicate GP1a, GP1b, GP1c, GP1d and GP1e. +, indicates positive reactivity; −, indicates no reactivity.
GP, glycoprotein; ELISA, enzyme-linked immunosorbent assay; MLR, mucin-like region; ND, not determined.