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. Author manuscript; available in PMC: 2018 Dec 11.
Published in final edited form as: Nat Med. 2018 Jun 11;24(8):1234–1245. doi: 10.1038/s41591-018-0059-x

Figure 6. Macrophage subpopulations are associated with outcome following mechanical unloading.

Figure 6

a, Immunostaining for CD68 (green) and CCR2 (red) in myocardial tissue specimens obtained from heart failure patients at the time of left ventricular assist device placement (pre-LVAD) and at the time of transplant (post-LVAD). Patients were stratified into those who displayed persistent LV systolic dysfunction (n=17) and those who displayed improved LV systolic function (n=18). Blue: DAPI, 200X magnification. b-c, Numbers of total (CD68+) (b) and CCR2+ (c) macrophages in pre-LVAD and post-LVAD specimens. Mann Whitney test (two-sided): CD68+ pre-LVAD, p=0.40 and CD68+ post-LVAD, p=0.16. d, Percentage of CCR2+ macrophages in pre-LVAD and post-LVAD. All data points represent biologically independent specimens and the lines indicate mean values. Asterisks indicate statistically significant p-values using Matt Whitney test (two-sided). e-f, Linear regression analysis for the association of the percentage of CCR2 macrophages and absolute changes in EF (e) and LV systolic dimension (f) over time. Dashed lines indicate 95% confidence intervals. Asterisks denotes p<0.05. 1-β denotes statistical power. Each data point (n=22) represents a biologically independent sample.