REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial Strains
Rosetta 2(DE3) E. coli strain	Novagen	Cat# 71397-3
Rosetta 2 (DE3) pLysS E. coli strain	Novagen	Cat# 71403
Chemicals, Peptides, and Recombinant Proteins
BS3 (bis(sulfosuccinimidyl)suberate)	Thermo Fisher Scientific	Cat# 21580
Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate)	Thermo Fisher Scientific	Cat# 22322
N,N-Dimethyl-p-phenylenediamine	Sigma Aldrich	Cat# 193992
Dithiothreitol (DTT)	US Biological	Cat# D8070
Sodium sulfide	Sigma Aldrich	Cat# 208043
L-Cysteine	Sigma Aldrich	Cat# W326305
4-(2-Hydroxyethyl)piperazin-1-ylethanesulfonic acid (HEPES)	VWR	Cat# 97061-826
Tris (2-Carboxyethyl) phosphine Hydrochloride (TCEP)	UBPBio	Cat# P1020-100
Iron(III) chloride	Sigma Aldrich	Cat# 157740
Trifluoroacetic acid (TFA)	Sigma Aldrich	Cat# T6508
Trypsin	Sigma Aldrich	Cat# T1426
Endoproteinase Glu-C	Sigma Aldrich	Cat# P6181
Human NFS1	This paper	Uniprot Q9Y697
Human ISCU	This paper	Uniprot Q9H1K1
Human ISD11	This paper	Uniprot Q9HD34
E. coli acyl carrier protein (Acp)	This paper	Cat# 21580
Human frataxin (FXN)	This paper	Cat# 22322
Oligonucleotides
Primers for ISCU: 5′:ACTAGTGGTACCGGTCTCAAGGT CTAAAAATGTTGGAACTGGACTGG 3′:GGGCCCGCTCGAGGGATCCTCATTA TTTCTTCTCTGCCTCTCCTTTTTTG	IDT https://www.idtdna.com/pages	N/A
Primers for NFS1: 5′:ACTAGTGGTACCGGTCTCAAGGT GGGCCAGTGCTGCGACCTCTCTATATG 3′:GGGCCCGCTCGAGGGATCCTCATTA GTGTTGGGTCCACTTGATGCT	IDT https://www.idtdna.com/pages	N/A
Primers for FXN: 5′:ACTAGTGGTACCGGTCTCAAGGT TCTGGAACTTTGGGCCACCCAGGC 3′:GGGCCCGCTCGAGGGATCCTCATTA TCAAGCATCTTTTCCGGAATAGGC	IDT https://www.idtdna.com/pages	N/A
Primers for ISD11: 5′:GAAGGAGATATACC ATGGCAGCCTCCAGTCGCGCACAA 3′:GGATCCTCGAGCTC TTAATGGTGATGGTGATGGTGTTTGGTCCTGGG CATGTCTCGATTC	IDT https://www.idtdna.com/pages	N/A
Recombinant DNA
cDNA of NFS1, ISD11, ISCU and frataxin	Mammalian Gene Collection	N/A
Plasmid: pET-SUMO, pET14b	This paper	N/A
Experimental Models: Constituents of Protein Complexes
Homo sapiens NFS1	This paper	UniProt: Q9Y697 (53-457)
Homo sapiens ISD11	This paper	UniProt: Q9HD34
Homo sapiens frataxin	This paper	UniProt: Q16595 (81-210)
Escherichia coli acyl carrier protein	This paper	UniProt: P0A6A8
Deposited Data
SAXS for (NIA)2	This paper	SASDB: SASDDB3
SAXS for (NIAU)2	This paper	SASDB: SASDDC3
SAXS for (NIAUF)2	This paper	SASDB: SASDDD3
NMR chemical shift data	This paper	BMRB: 27171
MS data	This paper	PRIDE: PSD006938, PSD006928, PSD009079
Structural model (NIAUF)2	This paper	PDBdev: PDB_DEV00000015
Software and Algorithms
ATSAS	(Franke et al., 2017)	https://www.embl-hamburg.de/biosaxs/software.html
DAMIF	(Franke and Svergun, 2009)	https://www.embl-hamburg.de/biosaxs/dammif.html
DAMAVER	(Volkov and Svergun, 2003)	https://www.embl-hamburg.de/biosaxs/damaver.html
CRYSOL from PRIMUS in ATSAS 2.8.1	(Svergun et al., 1995)	https://www.embl-hamburg.de/biosaxs/crysol.html
SUPCOMB	(Kozin and Svergun, 2001)	https://www.embl-hamburg.de/biosaxs/supcomb.html
Stavrox	(Gotze et al., 2012)	https://www.stavrox.com/
HADDOCK	(van Zundert et al., 2016)	https://haddock.science.uu.nl/services/HADDOCK2.2/
Mascot	Matrixscience	http://www.matrixscience.com/search_form_select.html
FoXS	(Schneidman-Duhovny et al., 2016)	https://modbase.compbio.ucsf.edu/foxs/
SAXS	Bruker	https://www.bruker.com/
ProtParam	(Wilkins et al., 1999)
PyMOL	Schrödinger	https://pymol.org/2/