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. 2001 Oct 30;98(23):13078–13083. doi: 10.1073/pnas.241337698

Figure 2.

Figure 2

Activator-specific interactions mediate activation by hybrid TAF (110-yST) and a IIB-AD fusion protein. (A) The two hybrids tested are depicted with their interaction partners, V = VP16 or VDR. (B) Expression vectors for CAD-G4, G4-VP16, and G4-VDR were transformed into Y-153 with the hybrid TAF protein or with the TFIIB-AD two-hybrid expression vector as indicated. β-Galactosidase activity was determined by using the o-nitrophenyl-β-d-galactopyranoside synthetic substrate assay. Data represent three independent transformations, with three colonies assayed from each.