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. Author manuscript; available in PMC: 2019 Jan 9.
Published in final edited form as: Nat Med. 2018 Jul 9;24(8):1246–1256. doi: 10.1038/s41591-018-0092-9

Figure 3.

Figure 3

Senescent cells reduce resilience to metabolic stress in mice. (a) Experimental design for transplantation and physical function measurements. (b-h) Maximal walking speed (relative to baseline) (b), hanging endurance (c), grip strength (d), daily activity (e), food intake (f), body weight change from baseline (g), and treadmill endurance (h) of 8-month-old male C57BL/6 mice 1 mo after being on HFD and injected with 0.4 × 106 SEN or CON preadipocytes (n = 6 for both groups). (i) Percent changes in RotaRod (on NCD, n = 21 for both SEN and CON; on HFD, n = 12 for both SEN and CON) and hanging test (on NCD, n = 6 for both SEN and CON; on HFD, n = 6 for both SEN and CON) in mice transplanted with 0.4-0.5 × 106 SEN cells relative to the average of mice transplanted with 0.4-0.5 × 106 CON cells. (j) Experimental design for transplantation and physical function measurements. (k-q) Maximal walking speed (relative to baseline) (k), hanging endurance (l), grip strength (m), body weight change from baseline (n), treadmill endurance (o), daily activity (p), and food intake (q) of 8-month-old male C57BL/6 mice 1 mo after being on HFD and injected with 1 × 106 SEN or CON autologous ear fibroblasts (n = 10 for both groups). All results are shown as box and whiskers plots, where a box extends from the 25th to 75th percentile with the median shown as a line in the middle, and whiskers indicate smallest and largest values. *P <0.05; Two-tailed unpaired Student’s t-tests (a-q).