Fig. 2.

N-terminal modifications of CENP-A facilitate CCAN recruitment. a NRMT1 trimethylates CENP-A at Gly-1 in interphase; the proportion of which is increased during mitosis. Aurora-A initiates the Ser-7 phosphorylation in prophase, the mark is then maintained by Aurora B. During anaphase, Ser-7 phosphorylation is reduced. b CENP-A trimethylation facilitates the recruitment of CCAN components CENP-T and CENP-I. Ser-7 phosphorylation mediates the recruitment of CENP-C through 14-3-3 proteins. The CENP-A C-terminal tail also directly recruits CENP-C independent of Aurora phosphorylation