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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Hepatology. 2018 May 2;68(2):691–706. doi: 10.1002/hep.29833

Figure 1. miR-155 deficiency aggravates liver injury in a ConA model.

Figure 1

Wild type and miR-155−/− mice were treated with physiological saline (WT, n=6; miR-155−/−, n=6) or with 10 mg/Kg of ConA (WT, n=5; miR-155−/− n=4). Animals were evaluated at 18 hours after ConA treatment. (A) miR-155 expression in livers of WT animals (B) AST, ALT and LDH levels were measured in serum (C) Representative images of livers analysed by TUNEL assay staining at X40 magnification, and quantification of the stained area; (n≥9 *p<0.05) with respect to WT-ConA treated animals (D) Protein levels of RIPK1, RIPK3, cleaved Caspase 3 and pJNK were determined by Western Blot in livers of WT and miR-155−/− ConA-treated animals. GAPDH was used as a loading control. (E) Hepatic gene expression of several inflammatory mediators was measured by qPCR and expressed as 2−ΔΔCt fold change vs. WT vehicle treated animals. Significant p values are shown above the bars: *p<0.05, **p<0.01 with respect to WT vehicle-treated animals.