Fig. 2.

PEGASOS passive immersion procedure clears both hard and soft tissue organs. a Brief description of the PEGASOS passive immersion procedure for clearing hard tissue organs. b Femurs, short vertebrae segment ( < 3 cm length) and mandible were harvested from adult mice (60 days of age) and imaged before and after clearing. Dotted lines outline the organs after clearing. Arrows indicate teeth before and after clearing. c Short vertebrae from Tie2-Cre; Ai14 mice was imaged through the bone to reveal the inside vasculatures. Boxed area is enlarged in c’. d Tooth of Tie2-Cre; Ai14 was imaged after clearing to reveal the vascular network within the dental pulp. Boxed area is enlarged in d’. e Brief description of the PEGASOS passive immersion procedure for clearing soft tissue organs. f Various soft tissue organs harvested from adult mice (60 days of age) were imaged before and after clearing. An intact liver lobe (g) and an intact kidney (h) harvested from adult Tie2-Cre; Ai14 mice (60 days of age) were imaged after clearing to reveal the vascular organization. Boxed areas are enlarged in g’ and h’, respectively. Arrows in h’ indicate renal capsules. Scale bars in b and f, 5 mm