Table 1.
Comparison of scRNA-seq library preparation methods
| Platform | Smart-seq | MARS-seq | CEL-seq | Drop-seq |
|---|---|---|---|---|
| Region | Full-length | 3′ end | 3′ end | 3′ end |
| Target read depth (per cell) | (106) | (104)–(105) | (104)–(105) | (104)–(105) |
| UMI | None | Yes | Yes | Yes |
| Amplification | PCR | IVT | IVT | PCR |
| Feature | Isoform analysis | FACS sorting Multiplex barcoding | Linear amplification (pool cDNAs for IVT) | Emulsion Low cost |
scRNA single-cell RNA sequencing, Smart-seq novel full-transcriptome mRNA-sequencing protocol, CEL-seq cell expression by linear amplification and sequencing, Drop-seq droplet sequencing, IVTin vitro transcription, UMIunique molecular identifier, FACSflow-activated cell sorting, MARS-seqmassively parallel RNA single-cell sequencing framework