Skip to main content
. 2018 Aug 8;8:11873. doi: 10.1038/s41598-018-30373-1

Figure 4.

Figure 4

Gene expressions in human psoriatic skin, and inhibitory effects of RvE1 on the IL-23 production by DCs and migration of DCs, Th17 and Tc17 cells. (a) Box and whisker plots of mRNA levels of IL23A, IL17A, LTB4R, LTA4H, and ALOX5. Box and whisker plots of Z scores (Y-axis) for skin taken from human healthy controls, and non-lesional and lesional skin taken from psoriasis patients in a public data set (GEO accession no. GSE13355). The length of the box represents the distance between 25% and 75%, and the horizontal line inside the box represents the group median. The whiskers indicate minimum and maximum values. All p-values were obtained by nonparameteric Wilcoxon-Mann-Whitney test. (b) The effects of LTB4 (100 nM), RvE1 (100 nM) and a BLT1 antagonist (10 nM) on IL-23p19 production by human monocyte-derived DCs. The amount of IL-23p19 in culture supernatant was measured by ELISA. (c) Transwell migration assay for human MoDCs, Tc17, and Th17 cells. Thirty minutes after RvE1 (100 nM), a BLT1 antagonist (U-75302) (10 nM), or vehicle treatment, human MoDCs, Tc17 and Th17 cells were tested for transmigration for 5 h (BMDCs) or 3 h (Tc17 and Th17 cells) in the presence of vehicle or LTB4 (10 nM) in the lower (BMDCs) or upper (Tc17 and Th17 cells) chamber. Results are expressed as the mean ± SD. All p-values were obtained by Student’s t-test: *p < 0.05. N.S indicates no significant difference. Data are representative of at least three independent experiments.