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. 2018 Aug 8;9:3164. doi: 10.1038/s41467-018-05550-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Decitabine treatment decreases the expression and the CGI methylation levels of genes hypermethylated and overexpressed in Alb-R26^Met tumours. Expression and CGI methylation levels of a set of genes found hypermethylated and overexpressed in the Alb-R26^Met tumours were analysed in dissected tumours from Alb-R26^Met mice either untreated (red) or treated with Decitabine (green). For each indicated gene, graphs report the methylation levels of CpGs within the CGI of interest (left) and the expression levels of genes (right) in tumours from Alb-R26^Met mice either untreated (red) or treated with Decitabine (green), compared to control livers (blue). Note that demethylating treatment significantly decreased transcription levels. Concerning the Scn8a gene, the methylation levels of its gene body CGI was reduced in Decitabine treated tumours compared to untreated tumours. This was accompanied by a trend in downregulation of its expression levels, although not significant. It is possible that for Scn8a, the demethylation extent caused by the dose of Decitabine used is suboptimal to significantly influence its expression levels. Alternatively, a more complex mechanism could be involved in the regulation of Scn8a expression. Significance is indicated on the top. Not significant (ns): P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001