Table 1.
Transgenic mice from retroviral vector transduction of mouse spermatogonial stem cells
| Exp. | Donor (age)* | Recipient mouse number† | Recipient (age)‡ | Testis weight, mg§
|
Colonies/testis¶
|
Area, mm2/testis‖
|
Transplant to first transgenic** | Transgenic/total progeny (%)‡‡ | |||
|---|---|---|---|---|---|---|---|---|---|---|---|
| R | L | R | L | R | L | ||||||
| 1 | B6/129 F2 (5 day) | 5483 | Wv/W54 (12 day) | 87.4 | 29.5 | 12 | 1 | 27.5 | 12.1 | 114 | 10/174 (5.7) |
| 5484 | Wv/Wv (5 day) | 25.9 | 47.3 | 3 | 9 | 4.7 | 21.3 | — | — | ||
| 5485 | Wv/W54 (12 day) | 22.6 | 23.1 | 4 | 0 | 4.3 | 0 | — | — | ||
| 5486 | Wv/W (5 day) | 74.4 | 48.0 | 12 | 1 | 12.0 | 0.3 | 114 | 8/111 (7.2) | ||
| 2 | B6 (5–7 day) | 5650 | B6/129 (7 day) | 144.7 | 130.7 | 0 | 0 | 0 | 0 | — | 0/103 (0) |
| 5651 | Wv/W54 (14 day) | 49.5 | 43.0 | 4 | 4 | 9.6 | 2.7 | 168 | 1/101 (1.0) | ||
| 5654 | Wv/W54 (8 day) | 35.8 | 50.0 | 1 | 1 | 2.6 | 3.3 | — | 0/95 (0) | ||
| 5655 | Wv/W54 (8 day) | 50.0 | 36.8 | 4 | 2 | 5.7 | 5.2 | 238 | 1/13 (7.7) | ||
| 3 | B6 (adult) | 5763 | B6/129 (8 day) | 227.6 | 92.4 | 0 | 0 | 0 | 0 | — | 0/100 (0) |
| 5764 | Wv/W54 (12 day) | 49.9 | 44.5 | 3 | 4 | 5.7 | 7.5 | 172 | 6/80 (7.5) | ||
| 5767 | Wv/W54 (10 day) | ND | 45.5 | — | 3 | — | 4.1 | — | 0/3 (0) | ||
| 5768 | B6/129 (7 day) | 143.7 | 135.5 | 0 | 0 | 0 | 0 | — | 0/103 (0) | ||
| 5769 | B6/129 (7 day) | 145.2 | 152.2 | 0 | 0 | 0 | 0 | — | 0/102 (0) | ||
Donor cell strain; adult, cryptorchid.
The number of cultured cells injected per recipient testis (1.2, 2.0, and 4.0 × 105 in Exps. 1, 2, and 3, respectively) was the adjusted number of the original cultured testis cell population introduced. Cultured cells (originally 6 × 106 testis cells) were recovered after 3 days and suspended in 100, 60, and 30 μl for Exps. 1, 2, and 3, respectively. Feeder cells cannot make colonies and were not included in calculations. Of each cell suspension, 2 μl were injected into a testis. Therefore, in Exp. 1, 6 × 106 cells per 100 μl × 2 μl injected per testis = 1.2 × 105 cultured testis cells were injected per recipient testis. All cells recovered from culture cannot be injected into a recipient testis because the seminiferous tubule volume and number of recipients are limited.
Strain of recipient mouse and approximate age in days at time of donor cell transplantation.
R, right; L, left; ND, not determined.
Number of individual blue colonies in each testis. Each colony generally represents the progeny of one donor stem cell. Totals for W recipients: Exp. 1 = 42; Exp. 2 = 16; Exp. 3 = 10.
Total blue surface area of all colonies in each testis that represents area of spermatogenesis expressing the transgene. Totals for W recipients: Exp. 1 = 82.2 mm2; Exp. 2 = 29.1 mm2; Exp. 3 = 17.3 mm2.
Time in days from transplantation of transduced donor cells to birth of first transgenic pup sired by the recipient.
Numerator is the number of transgenic progeny; denominator is the total number of progeny from each recipient. Totals for W recipients: Exp. 1 = 18/285 (6.3%); Exp. 2 = 2/209 (1.0%); Exp. 3 = 6/83 (7.2%).