Figure 4.

Polyplex Exposure Duration Does Not Contribute to Efficacy of Gene Expression and Biocompatibility in Astrocytes

Human astrocytes (150,000/well) were treated with S2 polymer:pDNA polyplexes (A–C) for 3 hr and washed or (D–F) left unwashed prior to testing. (A and D) Luciferase activity, (B and E) LDH activity, and (C and F) metabolic activity was measured at 3, 8, 24, and 48 hr post-treatment (A₁P₁₀ red line, A₅P₁₀ blue line, A₇P₁₀ green line). Data represent mean ± SEM for two donors with a minimum of triplicate determinations/donor (^$p < 0.05 for comparison to A₇P₁₀; ^#p < 0.05 for comparison to A₅P₁₀, *p < 0.05, **p < 0.01, ***p < 0.001 versus controls at a specific time point).