Table 1.
Physicochemical Characteristics of AnPn Polyplexes
| Polymer Name | MES-NHS Buffer pH | PEI Equivalents | Arginine Equivalents | PEG Equivalents | Polyplex Particle Size (nm) | Variance | Zeta potential (mV) |
|---|---|---|---|---|---|---|---|
| S1 A1P10 | not adjusted | 1 | 1 | 10 | 55.33 ± 0.80 | 0.249 ± 0.02 | 28.29 ± 0.16 |
| S1 A5P10 | not adjusted | 1 | 5 | 10 | 60.00 ± 1.64 | 0.297 ± 0.03 | 23.82 ± 0.29 |
| S1 A7P10 | not adjusted | 1 | 7 | 10 | 61.97 ± 1.07 | 0.214 ± 0.02 | 27.21 ± 1.45 |
| S2 A1P10 | pre-adjusted | 1 | 1 | 10 | 56.8 ± 0.43 | 0.211 ± 0.01 | 27.08 ± 1.32 |
| S2 A5P10 | pre-adjusted | 1 | 5 | 10 | 60.43 ± 0.05 | 0.252 ± 0.01 | 27.9 ± 0.86 |
| S2 A7P10 | pre-adjusted | 1 | 7 | 10 | 85.77 ± 7.55 | 0.332 ± 0.02 | 24.60 ± 3.12 |
Polyplex (polymer:pDNA) 2:1 w/w; MES, 2-(N-morpholino)ethanesulfonic acid; PEI, polyethylenimine; PEG, polyethylene glycol. Values represented as mean ± SEM.