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. 2018 Aug 2;9:1758. doi: 10.3389/fimmu.2018.01758

Figure 2.

Figure 2

Patient CD8+ T cells display elevated expression of inhibitory receptors and impaired killing of autologous targets. (A) Elevated expression of inhibitory receptors PD-1 and 2B4, and senescence marker CD57 on allo-reactive CD8+ T cells. Expression of the signaling lymphocyte activation molecule family receptor, NTB-A, remained unchanged, while SAP expression can be reduced. Representative example shown [3 healthy donor (HD) controls, 3–5 patients] [PT], small horizon line represents mean, *p < 0.05 (Mann–Whitney test). (B) Defects in Epstein–Barr virus (EBV)-specific CD8+ T cells. Cytolysis of P815 targets by anti-CD3-mediated redirected lysis (left panel) and cytolysis of peptide-pulsed autologous LCLs (middle panel), (cytolysis from 2 HD controls and 4 patients cytotoxic T lymphocytes done in duplicate are shown, representative of 4 independent experiments). Right panel: an example of cytolysis of healthy donor (HD) and patient (PT) peptide-pulsed autologous LCLs, with titration of effector:target ratios. (C) HD EBV-specific CD8+ T cells cytolysis of HD or PT LCLs. Two examples are shown, which are representative of three independent experiments.