Figure 6.

Rosuvastatin inhibited microglial activation. (A) Representative images of DAPI and Iba-1 staining, as described above. Scale bars: 50 μm. (B) The quantified Iba-1 immunofluorescence intensity at 24 h after reperfusion; the results were normalized to DAPI. (C) Transmission electron microscopy image showing the ultrastructure of microglia. Scale bar: 200 nm. (D) Iba-1 expression levels, as determined by western blotting (n = 6 in each group); the results are normalized to β-actin. The values were expressed as the mean ± SEM, the determination of which was followed by the LSD test (homogeneity of variance was determined; a–d for the SHAM, HT, HT+LRO and HT+HRO groups, respectively, as shown in Figure 1). *P < 0.05 and and ***P < 0.001 vs. the SHAM group, ^#P < 0.05, ^##P < 0.01 and ^###P < 0.001 vs. the HT group, ^&&P < 0.01 vs. the normal-dose rosuvastatin-treated group.