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. 2018 Aug 2;9:807. doi: 10.3389/fphar.2018.00807

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Copyright © 2018 Ho, Chen, Wang, Hsu, Chin, Yang, Wang, Chen, Shih, Liu, Wang, Whang-Peng, Tang, Lin, Liu and Lin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Anoectochilus formosanus extract regulates expression of anti-proliferative genes and cell proliferation in oral cancer SCC25 cells. SCC-25 cells were treated with different concentrations of AFE or metformin with refreshed media with agents daily for 3 days. (A) Total RNA was extracted and qPCR was performed for BAD and CAPS2. n = 3 ^∗∗∗p < 0.001 as compared with control. (B) SCC-25 cells were treated with AFE (0.2 and 1 mg/ml) or metformin (1 mg/ml) with refreshed media with agents daily for 3 days. AFE induced anti-proliferation. –: treated with normal saline as control; n = 3 ^∗p < 0.05, ^∗∗p < 0.01 as compared with control.