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. 2018 Aug 2;9:1050. doi: 10.3389/fphys.2018.01050

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Copyright © 2018 Di Cosmo, Bertapelle, Porcellini and Polese.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Example of the gating strategy for flow cytometry analysis. In this sample gating performed on OL neurons, cells were first gated for Propidium Iodide staining, to exclude debris (FL2-A vs. FSC-A, see panels A and D) and then gated for singlets (FL2-H vs. FL2-A, see panels B and E), to exclude cell doublets. This cell population was further analyzed for their uptake of BrdU versus DNA content (FL1-H vs. FL2A). Panels C and F show the gates used to evaluate the percentage of cells in S and G2/M phase (stained with PI) and to measure the percentage of BrdU-positive cells (DNA content ranging between G1 and G2).