Figure 1.

HA Expression Levels by HAd-H5HA or BAd-H5HA in Infected Cells

BHH3 cells were either mock infected or infected with HAd-ΔE1E3, HAd-H5HA, BAd-ΔE1E3, or BAd-H5HA at an MOI of 10 PFUs per cell. At 48 hr post-infection, the cells were harvested and processed for immunoblotting. HA expression was detected using an HA-specific polyclonal antibody. The expression levels of β-actin were monitored to demonstrate equal loading. The molecular weight markers in kDa are shown on the left.